{"id":3631,"date":"2024-06-11T00:45:14","date_gmt":"2024-06-10T15:45:14","guid":{"rendered":"https:\/\/www.kotai-bio.com\/service\/?page_id=3631"},"modified":"2026-06-15T10:48:44","modified_gmt":"2026-06-15T01:48:44","slug":"visium-hd","status":"publish","type":"page","link":"https:\/\/www.kotai-bio.com\/service\/detail\/visium-hd\/","title":{"rendered":"Visium HD WT\/3&#8242; \u7a7a\u9593\u7684\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u8a73\u7d30\u60c5\u5831"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"sample-condition\">\u30b5\u30f3\u30d7\u30eb\u53d7\u3051\u5165\u308c\u6761\u4ef6<\/h2>\n\n\n\n<ul class=\"wp-block-list\"><li>HE\u67d3\u8272\u30fb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3057\u3001\u7d44\u7e54\u69cb\u9020\u304c\u5d29\u308c\u3066\u3044\u306a\u3044\u3053\u3068<\/li><li>\u30ad\u30e3\u30d7\u30c1\u30e3\u30fc\u30a8\u30ea\u30a2\u306b\u5207\u7247\u304c\u9069\u5207\u306b\u8cbc\u308a\u4ed8\u3051\u3089\u308c\u3066\u3044\u308b\u3053\u3068<ul><li>\u5207\u7247\u306e\u8cbc\u308a\u4ed8\u3051\u4f4d\u7f6e\u306f\u4f7f\u7528\u3055\u308c\u308b\u30b9\u30e9\u30a4\u30c9\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u8a73\u3057\u304f\u306f\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044<\/li><li>\u30ad\u30e3\u30d7\u30c1\u30e3\u30fc\u30a8\u30ea\u30a2\u3088\u308a\u5927\u304d\u306a\u5207\u7247\u306e\u5834\u5408\u3001\u4e2d\u5fc3\u4f4d\u7f6e\u3092\u6307\u5b9a\u3057\u305f\u30b9\u30e9\u30a4\u30c9\u3092\u63d0\u51fa\u3059\u308b\u3053\u3068<\/li><\/ul><\/li><li>FFPE\u306e\u5834\u5408\u306f\u8584\u5207\u7247\u30925\u00b5m \u539a\u3067\u5207\u308a\u51fa\u3057\u3066\u3044\u308b\u3053\u3068<\/li><li>\u65b0\u9bae\u51cd\u7d50FF\u3001\u56fa\u5b9a\u51cd\u7d50FxF\u3067\u306f10\u00b5m \u539a\u3067\u5207\u308a\u51fa\u3057\u3066\u3044\u308b\u3053\u3068<\/li><li>10X genomics\u793e\u3067\u78ba\u8a8d\u6e08\u307f\u306e\u3044\u305a\u308c\u304b\u306e\u30ac\u30e9\u30b9\u30b9\u30e9\u30a4\u30c9\u306b\u5207\u7247\u3092\u8cbc\u308a\u4ed8\u3051\u3066\u3044\u308b\u3053\u3068<br> \u6ce8\uff09\u78ba\u8a8d\u6e08\u307f\u3067\u306f\u306a\u3044\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u3001\u5207\u7247\u304c\u5265\u304c\u308c\u3066\u3057\u307e\u3046\u5834\u5408\u304c\u3042\u308a\u307e\u3059<\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1740\" height=\"830\" src=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2025\/09\/image1.png\" alt=\"\" class=\"wp-image-5359\" srcset=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2025\/09\/image1.png 1740w, https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2025\/09\/image1-1536x733.png 1536w, https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2025\/09\/image1-640x305.png 640w\" sizes=\"auto, (max-width: 1740px) 100vw, 1740px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\"><li>\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eRNA\u306e\u54c1\u8cea\u304c\u9ad8\u3044\u3053\u3068<ul><li>\uff08\u5f0a\u793e\u3078\u59d4\u8a17\u53ef\u80fd\uff09FFPE\u304b\u3089\u8584\u7247\u3092\u5207\u308a\u51fa\u3057RNA\u62bd\u51fa\u3092\u884c\u3044\u3001FFPE\u306fDV200\u304c30%\u4ee5\u4e0a\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u3053\u3068\uff08Visium HD WT, FFPE\uff09<\/li><li>\uff08\u5f0a\u793e\u3078\u59d4\u8a17\u53ef\u80fd\uff09\u65b0\u9bae\u51cd\u7d50\u30d6\u30ed\u30c3\u30af\u304b\u3089\u8584\u7247\u3092\u5207\u308a\u51fa\u3057RNA\u62bd\u51fa\u3092\u884c\u3044\u3001RIN\u5024\u304c7\u4ee5\u4e0a\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u3053\u3068\uff08Visium HD 3\u2019, \u65b0\u9bae\u51cd\u7d50\uff09<br>\u305f\u3060\u3057\u3001Visium HD WT\u3092\u7528\u3044\u308b\u5834\u5408\u306f\u3001RIN\u5024\u304c4\u4ee5\u4e0a\u3067\u3042\u308b\u3053\u3068<\/li><li>\uff08\u5f0a\u793e\u3078\u59d4\u8a17\u53ef\u80fd\uff09In situ hybridization\uff08ISH\uff09\u306b\u3088\u308b\u7d44\u7e54\u5207\u7247\u4e0a\u3067\u306eRNA\u306e\u5206\u89e3\u7a0b\u5ea6\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u3053\u3068\uff08Visium HD WT, FFPE\uff09<\/li><\/ul><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>FFPE\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308b\u7cbe\u5ea6\u306e\u9ad8\u3044QC\u6cd5<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u300c\u7d30\u80de\u3042\u305f\u308a\u306eRNA\u91cf\u300d\u306e\u78ba\u8a8d\u306b\u3088\u308a\u3001\u4f4e\u691c\u51fa\u306b\u306a\u308b\u30ea\u30b9\u30af\u304c\u3042\u308bFFPE\u30b5\u30f3\u30d7\u30eb\u3092\u4e8b\u524d\u306b\u5224\u5b9a\u3057\u307e\u3059\u3002\u4ee5\u4e0b\u306e3\u30b9\u30c6\u30c3\u30d7\u306b\u3088\u308a\u3001\u6700\u9069\u306a\u30b5\u30f3\u30d7\u30eb\u9078\u5b9a\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u7b97\u51fa\u306e\u305f\u3081\u306e3\u30b9\u30c6\u30c3\u30d7<\/h4>\n\n\n\n<ul class=\"wp-block-list\"><li><strong>Step 1\uff1aRNA\u62bd\u51fa \uff06 DV200\u63a8\u5b9a<\/strong><ul><li>5\u00b5m\u306e\u5207\u72471\u679a\u5206\u306e\u7dcfRNA\u91cf\u3092\u7b97\u51fa\u3057\u307e\u3059\u3002<\/li><li>\u6ce8\u610f\uff1aDV200\u5024\u304c30%\u672a\u6e80\u306e\u30b5\u30f3\u30d7\u30eb\u306f\u975e\u63a8\u5968\u3068\u306a\u308a\u307e\u3059\u3002<\/li><\/ul><\/li><li><strong>Step 2\uff1aH&amp;E\u67d3\u8272 \uff06 \u5f62\u614b\u8a55\u4fa1<\/strong><ul><li>Step 1\u306e<strong>\u96a3\u63a5\u5207\u7247<\/strong>\uff085\u00b5m\u5207\u72471\u679a\uff09\u3092\u7528\u3044\u3066\u3001\u7d44\u7e54\u306e\u72b6\u614b\u3092\u8a55\u4fa1\u3057\u307e\u3059\u3002<\/li><li>\u6ce8\u610f\uff1a\u5f62\u614b\u4e0d\u826f\u3084\u91cd\u5ea6\u306e\u30a2\u30fc\u30c6\u30a3\u30d5\u30a1\u30af\u30c8\u304c\u3042\u308b\u30b5\u30f3\u30d7\u30eb\u306f\u975e\u63a8\u5968\u3068\u306a\u308a\u307e\u3059\u3002<\/li><\/ul><\/li><li><strong>Step 3\uff1a\u7d30\u80de\u3042\u305f\u308a\u306eRNA\u91cf\u7b97\u51fa<\/strong><ul><li>Step 2\u306eH&amp;E\u753b\u50cf\u3092\u57fa\u306b\u300110x Genomics\u793e\u306e\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2 <strong>Space Ranger\uff08v4.0\u4ee5\u4e0a\uff09<\/strong> \u306e <span style=\"color:#00d084\" class=\"tadv-color\">spaceranger segment<\/span> \u6a5f\u80fd\u3092\u6d3b\u7528\u3057\u3066\u6b63\u78ba\u306a\u7d30\u80de\u6570\u3092\u63a8\u5b9a\u3002\u4ee5\u4e0b\u306e\u8a08\u7b97\u5f0f\u304b\u3089\u7b97\u51fa\u3057\u307e\u3059\u3002<\/li><\/ul><\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"114\" src=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2026\/06\/image2.png\" alt=\"\" class=\"wp-image-6136\" srcset=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2026\/06\/image2.png 1024w, https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2026\/06\/image2-640x71.png 640w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"616\" height=\"242\" src=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2026\/06\/image4.png\" alt=\"\" class=\"wp-image-6137\"\/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\uff08\u5f0a\u793e\u59d4\u8a17\u53ef\u80fd\uff09<\/strong> \u5207\u7247\u4f5c\u6210\u3001H&amp;E\u67d3\u8272\u3001\u753b\u50cf\u8a55\u4fa1\u3001\u305d\u3057\u3066Space Ranger\u3092\u7528\u3044\u305f\u9ad8\u7cbe\u5ea6\u306a\u7d30\u80de\u6570\u30ab\u30a6\u30f3\u30c8\u304b\u3089RNA\u91cf\u7b97\u51fa\u306b\u3044\u305f\u308b\u307e\u3067\u3001\u4e00\u9023\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u3059\u3079\u3066\u5f0a\u793e\u3067\u304a\u5f15\u304d\u53d7\u3051\u3044\u305f\u3057\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u54c1\u8cea\u306b\u3054\u4e0d\u5b89\u304c\u3042\u308b\u5834\u5408\u3082\u3001\u307e\u305a\u306f\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u8a73\u7d30\u306b\u3064\u3044\u306610x Genomics\u793e\u306e\u30ea\u30f3\u30af\u3067\u53c2\u7167\u3067\u304d\u307e\u3059<ul><li>Visium HD WT<ul><li>FFPE\uff1a<a href=\"https:\/\/www.10xgenomics.com\/support\/spatial-gene-expression-hd\/documentation\/steps\/tissue-prep-for-ffpe\/visium-hd-ffpe-tissue-preparation-handbook\">Visium HD FFPE Tissue Preparation Handbook<\/a><\/li><li>\u56fa\u5b9a\u51cd\u7d50FxF\uff1a<a href=\"https:\/\/www.10xgenomics.com\/support\/spatial-gene-expression-hd\/documentation\/steps\/tissue-prep-for-fixed-frozen\/visium-hd-fxf-tissue-preparation-handbook\">Visium HD Fixed Frozen Tissue Preparation Handbook<\/a>\u3000<\/li><li>\u65b0\u9bae\u51cd\u7d50FF\uff1a<a href=\"https:\/\/www.10xgenomics.com\/support\/spatial-gene-expression-hd\/documentation\/steps\/tissue-prep-for-fresh-frozen\/visium-hd-ff-tissue-preparation-handbook\">Visium HD Fresh Frozen Tissue Preparation Handbook<\/a><\/li><\/ul><\/li><li>Visium HD 3\u2019\u00a0<ul><li>\u65b0\u9bae\u51cd\u7d50FF\uff1a<a href=\"https:\/\/cdn.10xgenomics.com\/image\/upload\/v1749046317\/support-documents\/CG000804_VisiumHD3__TissuePrepHandbook_RevA.pdf\">Visium HD 3\u2019 Fresh Frozen Tissue Preparation Handbook<\/a><\/li><\/ul><\/li><\/ul><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"data\">\u4e8b\u524d\u63d0\u51fa\u3044\u305f\u3060\u304f\u30c7\u30fc\u30bf<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u3054\u59d4\u8a17\u6642\u306b\u4ee5\u4e0b\u306e\u30c7\u30fc\u30bf\u3092\u78ba\u8a8d\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\u3002<br>\u3044\u305a\u308c\u3082\u5f0a\u793e\u306b\u59d4\u8a17\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>HE\u67d3\u8272\u753b\u50cf\uff1a\u30c0\u30e1\u30fc\u30b8\u306e\u5c11\u306a\u3044\u7d44\u7e54\u5207\u7247\u3067\u3042\u308b\u3053\u3068<\/li><li>\u7d44\u7e54\u306eDV200\u5024\uff1a30%\u4ee5\u4e0a\u3042\u308b\u3053\u3068<\/li><li>\u65b0\u9bae\u51cd\u7d50\u7d44\u7e54\u306eRIN\u5024\uff1aRIN\u5024\u22657\uff08Visium HD WT\u306e\u5834\u5408\u306f\u22654\uff09\u3067\u3042\u308b\u3053\u3068<\/li><li>\u7d30\u80de\u3042\u305f\u308a\u306eRNA\u91cf\u306f0.75 pg\u4ee5\u4e0a\u3067\u3042\u308b\u3053\u3068<\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">\u53c2\u8003\u753b\u50cf<\/h3>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2024\/06\/service__visium__sample1.png\" alt=\"\" class=\"wp-image-3737\"\/><\/figure><\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"https:\/\/www.kotai-bio.com\/service\/wp-content\/uploads\/2024\/06\/service__visium__sample2.png\" alt=\"\" class=\"wp-image-3738\"\/><\/figure><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"note\">\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u306e\u6ce8\u610f\u4e8b\u9805<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u306e\u969b\u306b\u306f\u4ee5\u4e0b\u306e\u70b9\u3092\u78ba\u8a8d\u306e\u4e0a\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u9001\u4ed8\u6642\u671f\u306b\u3064\u3044\u3066\u4e8b\u524d\u306b\u5f0a\u793e\u3078\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044<\/li><li>\u30b5\u30f3\u30d7\u30eb\u3092\u642d\u8f09\u3057\u305f\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u306f50mL\u9060\u6c88\u7ba1\u7b49\u306e\u5bc6\u9589\u5bb9\u5668\u306b\u5165\u308c\u3066\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044<\/li><li>\u9060\u6c88\u7ba1\u306b\u306f\u4e7e\u71e5\u5264\uff08\u30b7\u30ea\u30ab\u30b2\u30eb\uff09\u3092\u30b5\u30f3\u30d7\u30eb\u306b\u76f4\u63a5\u5f53\u305f\u3089\u306a\u3044\u3088\u3046\u306b\u5165\u308c\u3066\u304f\u3060\u3055\u3044<\/li><li>\uff08FFPE\u5207\u7247\u306e\u5834\u5408\uff09\u767a\u6ce1\u30b9\u30c1\u30ed\u30fc\u30eb\u5bb9\u5668\u306b\u4fdd\u51b7\u5264\u3068\u540c\u68b1\u306e\u3046\u3048\u30af\u30fc\u30eb\u4fbf\u306b\u3066\u304a\u9001\u308a\u304f\u3060\u3055\u3044<\/li><li>\uff08\u65b0\u9bae\u51cd\u7d50\u5207\u7247\u3001\u56fa\u5b9a\u51cd\u7d50\u5207\u7247\u306e\u5834\u5408\uff09\u767a\u6ce1\u30b9\u30c1\u30ed\u30fc\u30eb\u5bb9\u5668\u306b\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u3068\u540c\u68b1\u306e\u3046\u3048\u51b7\u51cd\u4fbf\u306b\u3066\u304a\u9001\u308a\u304f\u3060\u3055\u3044<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"peel-off\">\u5265\u304c\u308c\u3084\u3059\u3044\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u76ae\u819a\u3084\u8102\u80aa\u7d44\u7e54\u306e\u3088\u3046\u306a\u5265\u304c\u308c\u3084\u3059\u3044\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066\u306f\u4e8b\u524d\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<br>Matsunami Platinum Pro\u3088\u308a\u3082\u63a5\u7740\u6027\u306e\u9ad8\u3044Matsunami CREST\u3084\u3001\u5171\u6709\u7d50\u5408\u306e\u5f62\u6210\u306b\u3088\u308a\u5265\u96e2\u3092\u9632\u3050hydrogel coated slide\u306e\u4f7f\u7528\u306b\u3064\u3044\u3066\u4e8b\u524d\u691c\u8a0e\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"flow\">\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u30d5\u30ed\u30fc<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">10x Genomic\u793e\u306eVisium Spatial 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